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Human Rad51 filaments on double- and one-stranded DNA: correlating common and irregular sorts with recombination function.
Dynamics of initiation, termination and reinitiation of DNA translocation via the motor protein EcoR124I
Considering the fact that 2000, Dekker has shifted the leading target of his function to biophysics the place he research the Attributes of solitary biomolecules and cells utilizing the equipment of nanotechnology. This change of subject was driven by his fascination with the impressive functioning of Organic molecular structures, and also by the very long-term point of view that a lot of appealing discoveries may be envisioned Within this subject.[5] Recent strains of analysis in his biophysics team are within the regions of:[6]
This yielded a breakthrough. In just only a few months, we have been in the position to evaluate transport by means of someone solitary-walled carbon nanotube. Dependant on low-temperature experiments by a brilliant PhD pupil in my group, Sander Tans (now a valued colleague and professor at Delft College of Know-how having an Lively research lab at AMOLF in Amsterdam), we showed that carbon nanotubes had been real quantum wires with quite prolonged (micrometres) Digital coherence lengths — confirming theoretical predictions of metallic conduct.
Experimental section diagram of negatively supercoiled DNA calculated by magnetic tweezers and fluorescence,
You're a Christian. If you might make this type of synthetic mobile, would you be just one phase closer to God, or would the role of God have diminished to some degree yet again?
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‘The fantastically subtle way wherein things in character perform never ceases to amaze me. If we address the puzzle of how lifetime came into becoming, I hope that my amazement for the complexity of development may have amplified and with it my awe for that greatness in the Creator.’
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Nanoscientist Cees Dekker is definitely an amiable and flamboyant man who seems Substantially more youthful compared to the 62 decades he in fact is – trendy glasses, psychedelic shirt, participating smile – but not 1 to squander time. He usually takes his time, but not more than essential.
One example is, we consider the nucleopore complicated, a gateway concerning the mobile nucleus and also the cytoplasm. This is a really intricate program and therefore tricky to examine inside a mobile, so we use biomimetics. We reconstruct holes after which insert individual things in the nucleopore complex to review transport by way of it at the single-molecule stage.’
It had been an exceptionally interesting time, in the course of which I gained a lot of help from Delft College of Technological know-how, exactly where I used to be a professor at enough time, And that i begun several collaborations with molecular cees dekker biologists. That assisted me find out quite a bit.’